gene expression in rat astrocytes and microglial cells treated with lipopolysaccharide (LPS). Astrocytes and microglial cells were cultured and treated with vehicle or LPS (10 μg/ml) and the content of C/EBPβ and C3 proteins determined 24 hours later using specific antibodies, as indicated in Methods. (A) Representative western blot showing the levels of C/EBPβ and C3. Quantification analysis is shown. Values represent the mean ± SD of five different experiments. *P <0.05, **P <0.01. (B, C) Primary astrocytes (B) or microglial (C) cells were treated as commented above and the expression of C/EBPβ (red) and C3 (green) was evaluated using immunofluorescence techniques, as described in Methods. Nuclei were stained with DAPI.