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Figure 7 | Journal of Neuroinflammation

Figure 7

From: Chemokine-mediated inflammation in the degenerating retina is coordinated by Müller cells, activated microglia, and retinal pigment epithelium

Figure 7

Changes the number of CD45 + cells using fluorescence activated cell sorting (FACS), and their expression of chemokine receptors. A: Representative FACS plots, with gating strategies, for a 7-days postexposure sample stained for CD45. Gating methodology was applied equally for all samples. B: Histogram depicts changes in the population of retinal CD45+ cells following light damage (LD). Proportion of CD45+ cells roughly tripled following 24 hrs of LD (P <0.05), and continued to increase substantially during the post-exposure period after 3 days (P <0.05); a further increase at 7 days was not significant (P >0.05). The overall trend was significant by one-way ANOVA (P <0.05); n = 5. C-G: Representative images of PCR products via electrophoresis for Ccr1, Ccr2, Ccr5, Cxcr2, Cxcr3, in samples of CD45-sorted cells. Receptor expression was low in dim-reared control samples (C-F), and absent for Cxcr3 (G). Expression increased substantially following 24 hrs of LD for every receptor assessed (C-G), and was maintained through the post exposure period, with the exception of Cxcr3 (G).

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