Reduced TH17 frequency in patients with neuropathic pain. Using a multicolor flow cytometer, PBMCs were intracellularly stained with anti-human IL-17 antibody after five hours of stimulation. The results show a significantly decreased frequency of TH17 cells in the peripheral blood of patients suffering from neuropathic pain. In accordance with these results, the relative mRNA expression of the TH17 cell-specific transcription factor RORγT was reduced, but did not reach significance. T helper cells, TH; Peripheral blood mononuclear cells, PBMC; Interleukin, IL; RAR-related orphan receptor-γT, ROR-γT; *P <0.05 versus healthy controls.