GW0742 treatment did not reduce the number of neurons with intraneuronal APP/A
β. GW0742-treated mice showed less 6E10 immunoreactivity in cortical layer V (A) but the number of 6E10 positive neurons in cortical layer V was similar between the vehicle- and GW0742-treated mice (B). The relative intensity of intraneuronal 6E10 immunoreactivity was not altered by GW0742 treatment (C). Figures (D) and (E) depict typical examples of 6E10 immunoreactivity in cortical layer V of vehicle- (D) and GW0742- (E) treated mice. GW0742 treatment failed to significantly decrease the levels of brain APP (F) as analyzed by Western blotting. Figure (G) depicts a typical example image of APP expression levels in TG vehicle- and GW0742-treated mice. Results are presented as mean ± SEM. VEH = vehicle- and GW0742 = GW0742-treated mice. ***P < 0.001 as analyzed by Student’s t-test. N = 8 to 9 in vehicle- and n = 9 in GW0742-treated group. Scale bar = 200 μm.