GW0742 treatment prevented the loss of neurons in the subiculum. Figures (A -C) depict typical example images of NeuN positive neurons in the subiculum of WT vehicle- (A), TG vehicle- (B) and GW0742-treated (C) mice. NeuN immunopositive cells were manually counted (D) and the data were confirmed by quantifying the percentage of NeuN immunoreactive areas in the subiculum of the hippocampi (E). 5XFAD mice exhibited significant loss of NeuN positive cells, which was partially prevented by GW0742 treatment (D, E). Results are presented as mean ± SEM. VEH = vehicle- and GW0742 = GW0742- treated mice. **P < 0.01 ***P < 0.001 as analyzed by 1-way ANOVA followed by Tukey’s post hoc test. n = 6 in WT vehicle-treated group, n = 8 in TG vehicle- and n = 7 in TG GW0742-treated group. Scale bar = 200 μm.