Treatment with GW0742 did not alter the lipidated ApoE levels. The protein expression levels of Abca1 (A) were unaltered in vehicle-treated TG mice compared to their WT controls whereas the levels of Abcg1 (B) were significantly increased. There were no significant changes in the levels of ApoE between the TG vehicle- and WT vehicle-treated mice (C). Two-week treatment with GW0742 slightly reduced the levels of Abca1 (A) and the decrease was statistically significant compared to WT vehicle-treated mice but led to significant decrease in Abcg1 compared to TG vehicle-treated mice (B). The levels of ApoE were unaltered by GW0742 (C). Figure (D) depicts typical example images of Western blots against Abca1, Abcg1, ApoE and actin as a loading control. TG mice exhibited increased levels of lipidated ApoE in the brain compared to WT mice and the levels were unaltered by GW0742 treatment (E). Figure (F) depicts a typical example of the levels of lipidated ApoE (F). Results are presented as mean ± SEM. VEH = vehicle- and GW0742 = GW0742-treated mice. *P < 0.05 **P < 0.01 as analyzed by Student’s t-test or 1ne-way ANOVA followed by Tukey’s post hoc test. For Abca1, n = 5 in WT vehicle-treated group, n = 6 for TG vehicle-treated group and n = 7 for TG GW0742-treated group. For Abcg1, n = 4 in WT vehicle-treated group, n = 6 for TG vehicle-treated group and n = 8 for TG GW0742-treated group. For ApoE, n = 7 in WT vehicle-treated group, n = 9 in TG vehicle- and n = 11 in TG GW0742-treated group. For ApoE lipidation blot, n = 10 in WT vehicle-treated group, n = 9 for TG vehicle-treated group and n = 9 for TG GW0742-treated group.