Figure 3

Leukocyte vascular adhesion. Animals were treated with vehicle or FTY720 (0.5 mg/kg) 3 hours post-surgery and leukocyte adhesion was determined at 48 hours. (A) Representative pictures of the vessel anatomy (bright field) and the trafficking of leukocytes (dark field) 1 hour after leukocyte labeling with R6G in sham-vehicle, sham-FTY720, SAH-vehicle, and SAH-FTY720-treated animals. (B) Quantification of leukocyte adhesion, expressed as the percentage of the vessel area occupied by adherent leukocytes measured 1 hour after leukocyte labeling. Bars represent means ± SEM. n = 6 each group. Significance was determined using ANOVA. SAH, subarachnoid hemorrhage.