LPS treatment alters LB number, increases LB size and causes microglia hypertrophy in organotypic hippocampal slice cultures and N9 microglial cells. A Examples of LB formation in microglia from control and treated slices. In the presence of LPS (100 ng/ml or 1 μg/ml), more larger (>1.0 μm) LBs are present compared to control conditions. Microglial morphologies are also more ramified and appear to be more activated in the LPS-treated cultures. Scale bar 10 μm. Green, Iba-I. Red, ADRP. B LB density under various treatments with various LB sizes in microglia. *p < 0.05. C Examples of LB formation in N9 microglial cells showing that LPS treatment altered LB distribution. Following LPS treatment, there is an increase in LB formation. D LB densities and sizes following LPS treatment, showing that there is an increase in the large-sized LBs. *p < 0.05.