Figure 5
Effects of intrathecal injection of mimic-27a and AMO-27a on the expression and colocalization of TLR 4 and TICAM-2 in vivo after spinal cord ischemia reperfusion (IR). (a) Representative micrographs showing the colocalization of TLR4 (green) and TICAM-2 (Red) at 24 and 72 hours after IR injury. Arrows show their co-localization. Scale bars = 100 μm. (b) Quantification of TICAM-2 immunoreactivity is presented as the average fluorescence intensity (FI) of three independent experiments. (c) Histogram for the quantification of co-localized cells (cells with yellow signals). Double fluorescence immunohistochemistry showing that membrane-bound TLR4 has an expression profile that is similar to that of TICAM-2 in the cytoplasm of neurons and glial cells from injured regions of the spinal cord at 24 and 72 hours after surgery. Increasing the levels of miR-27a by intrathecal pretreatment with mimic-27a significantly decreased TICAM-2 immunoreactivity after IR and the number of TICAM-2-TLR4 positive cells, whereas these effects were reversed by intrathecal injection of AMO-27a. All data are presented as mean ± SEM. **P <0.01 versus the sham group; ## P <0.05 versus the IR group.