Figure 7

LXR activation is associated with delay in onset of EAE and induction of reverse cholesterol transport genes in the CNS. Animals induced to undergo EAE were administered daily intraperitoneal injections of GW3965 (GW) or vehicle (VC) beginning day 8 post-induction (arrow). Animals were killed at day 14 to obtain lymph nodes, spleen and spinal cord. All animals received daily injections up to 14 days post induction (N = 19 for the GW group, N = 22 for VC group). A subset of animals (N = 9 for the GW group, N = 12 for the VC group) received daily injections up to 21 days post induction. A Clinical score (mean ± SE) of EAE over time for GW and VC treated animals. B The frequency interleukin-17A positive (IL17+) and/or interferon-γ positive (IFNg+) CD3 + CD4+ T cells in the lymph nodes (cervical and axillary) of GW and vehicle VC treated animals at day 14 post induction. C CNS expression of reverse cholesterol transport genes (Abca1 and Abcg1) and inflammatory genes (Nos2 and Il1b) for VC and GW treated animals. Line and error bars indicate median with interquartile range. Mann–Whitney test of significance.