Table 3 Primer sequences for qPCR analysis
Gene | Abbreviations | Primer sequences | Accession |
|---|---|---|---|
Toll-like receptor 4 | TLR4 | F: ATTGCTCAGACATGGCAGTTTC | NM_019178.1 |
R: CACTCGAGGTAGGTGTTTCTGCTAA | |||
Wingless type MMTV integration site family, member 3A | WNT3A | F: TGCAAATGCCACGGACTATC | NM_001107005.2 |
R: AGACTCTCGGTGTTTCTCTACC | |||
Frizzled 9 | FZD9 | F: TACCCAGAGCGCCCTATAAT | NM_153305.1 |
R: CAAACCCTCCTGGATCACATAC | |||
Axis inhibition protein 2 | AXIN2 | F: ACCTATGCCTGTCTCCTCTAAC | NM_024355.1 |
R: GTCCAGGGTATCCACACATTTC | |||
Myelocytomatosis proto-oncogene | CMYC | F: CTTGGAACGTCAGAGGAGAAA | NM_012603.2 |
R: GCTTGAACGGACAGGATGTA | |||
Jun proto-oncogene | CJUN | F: GAAGCAGAGCATGACCTTGA | NM_021835.3 |
R: CCATTGCTGGACTGGATGAT | |||
Interleukin-1β | IL1B | F: AGTGAGGAGAATGACCTGTTC | NM_031512.2 |
R: CGAGATGCTGCTGTGAGATT | |||
Interleukin-6 | IL6 | F: GCCAGAGTCATTCAGAGCAATA | NM_26744.1 |
R: GTTGGATGGTCTTGGTCCTTAG | |||
Interleukin-10 | IL10 | F: ATTGAACCACCCGGCATCTA | NM_012854 |
R: CAACGAGGTTTTCCAAGGAG | |||
Tumor necrosis factor α | TNFa | F: GCAGATGGGCTGTACCTTATC | NM_012675.3 |
R: GGCTGACTTTCTCCTGGTATG | |||
Hypoxanthine-guanine phosphoribosyltransferase | HPRT | F: GCTGACCTGCTGGATTACAT | NM_012583.2 |
R: CCCGTTGACTGGTCATTACA |