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Figure 2 | Journal of Neuroinflammation

Figure 2

From: miR-146a suppresses cellular immune response during Japanese encephalitis virus JaOArS982 strain infection in human microglial cells

Figure 2

JEV downregulates TRAF6, IRAK1, IRAK2, and NF-κB activation. CHME3 cells were infected by JEV (MOI-5) and harvested 24 h post infection. (A) Western blots showing downregulation of TRAF6, IRAK1, and IRAK2 genes. Uninfected cells were used as control. (B) Graph bars representing densitometry plot depicting downregulation of TRAF6, IRAK1, and IRAK2 genes post JEV infection. The image density of blots was normalized by β-tubulin by using ImageJ software. (C) Western blot showing downregulation of phospho-p65 upon JEV infection. (D) Densitometry plot showing decreased phosphorylation of p65 subunit of NF-κB. The ratio of phospho/total p-65 decreases upon JEV infection. (E) Graph bar showing increased NF-κB luciferase activity at 6 and 12 h after JEV infection which later decreased 24 h post JEV infection. One-way ANOVA was used to determine statistical significance. P values were considered significant where *denotes P < 0.05, **denotes P < 0.005, and ***denotes P < 0.001 from 6 h sample, #P from 12 h sample. NF-κB luciferase vector was co-transfected with β-galactosidase vector. β-galactosidase activity was used to normalize luciferase activity. All experiments were repeated thrice and are represented as mean ± SE.

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