Figure 6

miR-146a suppresses ISRE promoter activity. CHME3 cells were co-transfected with 1 μg ISRE luciferase vector and 700 ng β-galactosidase vector to measure ISRE luciferase activity. (A) Graph bars showing reduced ISRE luciferase activity upon JEV infection in miR-146a overexpressing cells. miR-146a mimic was transfected along with vectors, and scrambled sequence was used as control for statistical analysis. Scrambled + JEV group did not show any significant decrease in ISRE activity upon JEV infection. JEV infection was given 24 h post transfection, and luciferase activity was measured after 24 h. (B) Graph bars showing increased ISRE luciferase activity upon JEV infection in anti-miR-146a transfected cells. Cy3-labeled scrambled anti-miR was used as control. Anti-miR-146a increased ISRE activity upon JEV infection as compared to scrambled Cy3 + JEV group. (C) Graph bars showing ISRE luciferase activity upon JEV infection at two time points - 12 and 24 h post infection. The ISRE activity decreases at later time point after JEV infection. All experiments were repeated thrice and are represented as mean ± SE. The fold change is significant where *denotes P < 0.05, **denotes P < 0.005, and ***denotes P < 0.001.