Figure 2

Involvement of σ-1R in methamphetamine-mediated σ-1R expression in primary rat astrocytes. (A) Pretreatment of primary rat astrocytes with the σ-1R antagonist-BD1047 (10 μM) inhibited methamphetamine-mediated increased expression of σ-1R. Representative immunoblots and the densitometric analysis of σ-1R/GAPDH from the three separate experiments are presented. (B) Immunofluorescent staining for σ-1R (green) and GFAP (red) in primary rat astrocytes treated with methamphetamine or σ-1R antagonist-BD1047 (10 μM). Scale bars all indicated 10 μm. (C) Intensity of σ-1R immunofluorescence was quantified in five areas of the slide using the Image J software. IOD-integrated optical density. All the data are presented as mean ± SD of three individual experiments. *P < 0.05 and **P < 0.01 versus control group; #P < 0.05 versus methamphetamine-treated group.