Figure 3

Effect of methamphetamine on Src activation. (A) Methamphetamine induced Src phosphorylation in a time-dependent manner in primary rat astrocytes. (B) Pretreatment of primary rat astrocytes with σ-1R antagonist-BD1047 (10 μM) inhibited methamphetamine-induced expression of p-Src. Representative immunoblots and the densitometric analysis of p-Src/Src from the three separate experiments are presented. (C) Immunofluorescent staining for p-Src (green) in primary rat astrocytes treated with methamphetamine and σ-1R antagonist-BD1047 (10 μM). Scale bars - 10 μm. (D) Fluorescent intensity of p-Src was quantified in five areas on the slide using the Image J software. IOD-integrated optical density. (E) Methamphetamine induced the interaction between σ-1R and p-Src using immunoprecipitation assay. All the data are presented as mean ± SD of three individual experiments. *P < 0.05 and **P < 0.01 versus control group; #P < 0.05 and ##P < 0.01 versus methamphetamine-treated group.