Figure 4

Methamphetamine-mediated activation of Src involves activation of ERK MAPK pathway. (A) Western blot analysis of time-dependent activation of ERK MAPKs by methamphetamine in primary rat astrocytes. (B) Pretreatment of primary rat astrocytes with σ-1R antagonist-BD1047 (10 μM) or Src inhibitor-PP2 (10 μM) but not its inactive analog PP3 (10 μM), resulted in inhibition of methamphetamine-mediated phosphorylation of ERK. Representative immunoblots and the densitometric analysis of p-ERK/ERK from the three separate experiments are presented. All the data are presented as mean ± SD of three individual experiments. *P < 0.05 versus control group; #P < 0.05 versus methamphetamine-treated group.