Table 1 Primers and probe to use for real-time PCR
Name | Official symbol | Species | Forward (5′ to 3′) | Reverse (5′ to 3′) | System |
|---|---|---|---|---|---|
PACAP | Adcyap1 | mouse | AACCCGCTGCAAGACTTCTATGAC | TTAAGGATTTCGTGGGCGACA | Cyber green (TaKaRa) |
PAC1R | Adcyap1r1 | mouse | GGCTGTGCTGAGGCTCTACTTTG | AGGATGATGATGATGCCGATGA | |
IL-1β | Il1b | mouse | TCCAGGATGAGGACATGAGCAC | GAACGTCACACACCAGCAGGTTA | |
TNFα | Tnf | mouse | GTTCTATGGCCCAGACCCTCAC | GGCACCACTAGTTGGTTGTCTTTG | |
IL-10 | Il10 | mouse | GACCAGCTGGACAACATACTGCTAA | GATAAGGCTTGGCAACCCAAGTAA | |
TGFβ1 | Tgfb1 | mouse | GTGTGGAGCAACATGTGGAACTCTA | TTGGTTCAGCCACTGCCGTA | |
IL-4 | Il4 | mouse | TCTCGAATGTACCAGGAGCCATATC | AGCACCTTGGAAGCCCTACAGA | |
RPLP1 | Rplp1 | mouse | TCCGAGCTCGCTTGCATCTA | CAGATGAGGCTCCCAATGTTGA | |
PACAP | ADCYAP1 | human | GTGAGGTAAGCAAGCTCCAACAGAC | CTCGATCTGATTGCTGGGTGAA | Cyber green (TaKaRa) |
PAC1R | ADCYAPR1 | human | CTCACCACTGCCATGGTCATC | GCCCTCAGCATGAACGACAC | |
NGF | NGF | human | AGCGTCCGGACCCAATAACA | CCTGCAGGGACATTGCTCTC | |
BDNF | BDNF | human | GTCAAGTTGGGAGCCTGAAATAGTG | AGGATGCTGGTCCAAGTGGTG | |
NT-3 | NTF3 | human | GAAACGGTACGCGGAGCATAA | GTCGGTCACCCACAGACTCTCA | |
IL-4 | IL4 | human | AGCAGCTGATCCGATTCCTGA | TCCAACGTACTCTGGTTGGCTTC | |
IL-10 | IL10 | human | GAGATGCCTTCAGCAGAGTGAAGA | AGTTCACATGCGCCTTGATGTC | |
TGFβ1 | TGFB1 | human | GCGACTCGCCAGAGTGGTTA | GTTGATGTCCACTTGCAGTGTGTTA | |
β2-microgloblin | B2M | human | CGGGCATTCCTGAAGCTGA | GGATGGATGAAACCCAGACACATAG | |
TSG-6 | TNFAIP6 | human | AAGCAGGGTCTGGCAAATACAAGC | ATCCATCCAGCAGCACAGACATGA | Taqman (Japan Bio Service) |
probe:FAM-TTTGAAGGCGGCCATCTCGCAACTT-TAMRA | Â | ||||