Immunization with cyclo-(87-99)[A
significantly decreases mechanical pain hypersensitivity following CCI. (A-D) Time course of mechanical withdrawal threshold of the hindpaws (in grams) in nerve-injured and sham-operated rats treated with MBP87-99 (native peptide), APL cyclo-(87-99)[A91
,A96]MBP87-99 and CFA only (vehicle control) in (A) the ipsilateral hindpaw and (B) the contralateral hindpaw of the rats with CCI and (C) ipsilateral hindpaw and (D) contralateral hindpaw of the sham-operated rats. +(P < 0.05), ++(P < 0.01), and +++(P < 0.001) represent significant differences between the MBP- and APL-treated rats, *(P < 0.05), **(P < 0.01), ***(P < 0.001), and ****(P < 0.0001) indicate significant differences between the APL-treated and control rats. #(P < 0.05) and ##(P < 0.01) represent significant differences between MBP-treated and control rats (n = 6 per group; mean ± SEM; two-way RM-ANOVA with Tukey’s multiple comparison test). Arrows indicate the time point of CCI/sham and immunization.