Figure 3

Immunohistochemistry of T cells in the sciatic nerve, DRGs, and spinal cord. The number of TCRαβ immunoreactive cells at the site of injury, proximal to the injury and distal to the injury in the left sciatic nerve (LSN) and uninjured right sciatic nerve (RSN) at 10 days (A) and 30 days (B) post-CCI. The number of TCRαβ immunoreactive cells in the L4 and L5 ipsilateral DRG (L-DRG) and contralateral DRG (R-DRG) at 10 days (C) and 30 days (D) post-CCI. The number of TCRαβ immunoreactive cells in the ipsilateral (left) and contralateral (right) lumbar spinal cord at 10 days (E) and 30 days (F) post-CCI. ^## P < 0.01 represents a significant difference between the APL-treated and control rats at the site of injury at 10 days post-CCI in the LSN. ****P < 0.0001 denotes significant differences between MBP-treated rats as compared to the APL-treated and control rats at 30 days post-CCI at the site of injury (n = 4 per group; mean ± SEM; two-way ANOVA with Tukey’s multiple comparison test). Representative images of (G) T cells in the uninjured nerve, (H) T cells in control, (I) T cells in MBP-treated and (J) T cells in APL-treated LSN at the site of injury. Scale bar = 50 μm.