Immunohistochemistry of ED1+ macrophages in the sciatic nerve. The average of ED1+ area density at the site of injury, proximal to the injury and distal to the injury in the left sciatic nerve (LSN) and right uninjured nerve (RSN) at 10 days (A) and 30 days (B) post-CCI. *P < 0.05 represents significant difference between APL-treated and control rats at the site of injury and +
P < 0.05 between MBP- and APL-treated rats distal to the injury at 10 days post-CCI. At 30 days post-CCI, *P < 0.05 represents significant difference between the APL and control group and +
P < 0.05 between MBP and APL group proximal to the injury. ****P < 0.0001 represents significant differences between the APL and control group, ++++
P < 0.0001 between MBP and APL group at the site of injury and distal to the injury; (n = 4 per group; mean ± SEM; two-way ANOVA with Tukey’s multiple comparison test). Representative images of (C) ED1+ cells in uninjured RSN, (D) ED1+ cells in CFA-treated control, (E) ED1+ cells in MBP-treated, and (F) ED1+ cells in the APL-treated LSN at the site of injury. Scale bar = 50 μm.