Immunohistochemistry of microglia and astrocytes in the lumbar spinal cord following CCI and immunization. The percentage of Iba-1+ area density in the dorsal and ventral lumbar spinal cord at 10 days (A) and at 30 days (B) post-CCI and the percentage of GFAP+ area density in the dorsal and ventral lumbar spinal cord at 10 days (C) and at 30 days (D) post-CCI. Representative images of Iba-1+ cells in the spinal cord of the control (E), MBP-treated (F), APL-treated (G), and GFAP+ cells in the spinal cord of the control (H), MBP-treated (I), and APL-treated (J) rats. +
P < 0.05 and ++
P < 0.01 represent significant differences between the MBP-treated and control rats, and **P < 0.01 and ****P < 0.0001 indicate significant differences between MBP- and APL-treated rats at 30 days post-CCI (n = 4 per group; mean ± SEM; two-way ANOVA with Tukey’s multiple comparison test).