Flow cytometry analysis of CD4+ CD25+ FoxP3+ expressing Treg cells in the spleen and lymph nodes following CCI and immunization. (A) Representative forward scatter (FSC) vs. side scatter (SSC) plot showing the acquired events and lymphocyte gate from rat spleen. (B) Representative histogram of counts vs. CD4+ cell population selected for further gating. (C) Representative dot plots of Treg cells in the spleen of the control (left), MBP-treated (middle), and APL-treated (right) rats at 10 days post-CCI. (D) The percentage of CD4+ CD25+ FoxP3+ cells in the spleen and lymph nodes at 10 and 30 days post-CCI. **P < 0.01 indicates significant differences between APL-treated and MBP-treated and control rats at 10 days post-CCI (n = 4 per group; mean ± SEM; two-way ANOVA with Tukey’s multiple comparison test).