Figure 5
Effect of thymic stimulation by castration on M1 and M2 microglial activation in spinal cords of male SOD1 G93A mice. Immunohistochemical analysis of lumbar spinal cords of sham and castrated (Cx) SOD1G93A mice at 120 days for (A) iNOS and (B) Arg1. M1 microglial activation shown by iNOS in sham SOD1G93A mice is reduced by castration. M2 microglial activation shown by Arg1 in sham SOD1G93A mice is increased by castration. Microglia were identified by Iba1 (not shown). Scale bar = 20 μm. Representative photomicrographs from two mice per group are shown.