Microglia expressed multiple NFAT isoforms. Microglia were grown as mixed glial cultures from postnatal day 0 C57BL6/J mice and purified at day 14 in vitro. Lysates of microglia (M) were compared to lysates from the Jurkat human T cell line (J) via Western blot using anti-NFATc1, c2, c3, c4, α-tubulin, and ERK-2 (loading control) antibodies. Multiple bands per NFAT isoform likely reflect the multiple alternative transcripts reported for each isoform. Blots are representative of three independent experiments.