Figure 3

FK506 stimulated increased microglial uptake of Aβ fibrils. Microglia were plated in the absence or presence of 1 μM FK506 for 24 h. Either 500 nM FITC-conjugated Aβ1-42 fibrils or 0.25 mg/mL FITC-conjugated bioparticles were added to the cells for an additional 6 h. After incubation, the media was removed and the signal from unphagocytosed peptide was quenched with trypan blue. Fluorescence intensity from phagocytosed peptide and bioparticle was quantitated using a fluorescent plate reader (480-nm excitation and 520-nm emission) and averaged (±SD). Conditions were performed with eight repeats per condition and graphs are representative of three independent experiments (±SD) (*p < 0.001 from control, ^# p < 0.05 from Aβ, ^% p < 0.001 from bioparticle).