Figure 2

LPS-induced increase in expression and activity of MEF2D in BV2 cells. (A) LPS-induced increase in MEF2D mRNA expression in BV2 cells. BV2 cells were exposed to 1.0 μg/ml LPS for 6, 12, 18, or 24 h, and the mRNA levels of MEF2D were quantified by qPCR and normalized to β-actin mRNA level as described in ‘Methods’. Data from three independent experiments were expressed as the mean ± SEM and analyzed by one-way ANOVA (**P < 0.01). (B) LPS-induced increase in MEF2D protein in BV2 cells. BV2 cells were treated for the indicated time as described above and immunoblotted for MEF2D. (B bottom graph) Relative quantification of MEF2D. Data were expressed as mean ± SEM from at least three independent experiments and analyzed by one-way ANOVA (**P < 0.01). (C) LPS-induced increase in MEF2D activity. The whole cell extracts were prepared from BV2 cells treated with or without LPS for stimulating 24 h and analyzed by EMSA. Arrow indicates the position of MEF2D-probe complex (unlabelled probe: a 100-fold excess of unlabelled probe was added in the reaction; MT probe: probe with MEF2 binding site mutated).