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Figure 5 | Journal of Neuroinflammation

Figure 5

From: Transcription factor myocyte enhancer factor 2D regulates interleukin-10 production in microglia to protect neuronal cells from inflammation-induced death

Figure 5

The effect of knocking-down MEF2D on microglia-mediated cytotoxicity. (A) Experiment paradigm for the collection of conditioned media (CM). Media collected from BV2 cells treated with LPS for 12 h were labeled as pre-12 h CM. After the first 12 h, cells were washed and placed in fresh media for another 12 h (post-12 h CM). CM was then added to SN4741 cells for 48 h. (B) The effect of silencing MEF2D on CM-mediated toxicity. CM was collected from BV2 cells infected with either control (sh-Control) or sh-MEF2D lentivirus (sh-MEF2D). The viability of SN4741 cells was determined by MTT. Data from three independent experiments were expressed as the mean ± SEM and analyzed by two-way ANOVA (**P < 0.01). (C) The effect of neutralizing IL-10 on CM-mediated toxicity. CM was collected from BV2 cells with (anti-IL-10+) or without (anti-IL-10−) anti-IL-10 neutralizing antibody. The viability of SN4741 cells was determined by MTT. Data from three independent experiments were expressed as the mean ± SEM and analyzed by two-way ANOVA (**P < 0.01). (D) The effect of silencing MEF2D or neutralizing IL-10 on CM-induced toxicity. For (D) left panel, the effect of CM from BV2 cells treated with LPS on SN4741 viability was assessed by TUNEL staining. For (D) middle panel, the effect of post-12 h CM collected from BV2 cells treated as in (B) or (C) were assessed by TUNEL assay in SN4741 cells. For (D) right panel, the quantification of the percentages of TUNEL-positive population. Data from three independent experiments were expressed as the mean ± SEM and analyzed by one-way ANOVA (**P < 0.01).

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