Figure 9

The BET family bromodomain inhibitor JQ1 reduces LPS-induced release of pro-inflammatory mediators. Primary microglial cell culture supernatants of LPS and/or JQ1 co-treated cells were subjected to ELISA to detect the levels of pro-inflammatory cytokines/chemokines. Therefore, primary microglial cells were treated with 10 ng/mL LPS and/or 500 nM of JQ1 for 2 and 4 h, followed by quantification of Ccl2, Ccl7, and Cxcl10 levels. Values are given in pg/ml. Means and standard deviations of the mean of the three independent experiments are shown (*P value <0.05, **P value <0.001). LPS, lipopolysaccharide; Con., control.