Figure 7

Minocycline neuroprotection to OGD-induced neuronal damage via MCPIP1 in primary cortical neurons from wild type mice. (A) A representative Western blot shows the induced expression of MCPIP1 by minocycline and the knockdown of MCPIP1 by siMCPIP1 in primary cortical neurons from C57BL/6 wild type mice. OGD induced MCPIP1 mildly, and minocycline induced MCPIP1 to a much higher level. siMCPIP1 knocked down MCPIP1 very efficiently. (B) MTT cell viability analysis shows that pretreatment with minocycline increased the resistance to OGD-induced neuronal damage and that knockdown of MCPIP1 by siMCPIP1 reduced the protective effects of minocycline on primary cortical neurons subjected to OGD. (C) Knockdown of MCPIP1 significantly increased the expression of TNFα induced by OGD in primary cortical neurons pretreated with minocycline compared with that of control group of siControl. MCPIP1, monocyte chemotactic protein-induced protein 1; MTT, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide; OGD, oxygen glucose deprivation; siControl, negative control silencing RNA; siMCPIP1, MCPIP1 silencing RNA.