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Figure 2 | Journal of Neuroinflammation

Figure 2

From: miR-15b/16 protects primary human retinal microvascular endothelial cells against hyperglycemia-induced increases in tumor necrosis factor alpha and suppressor of cytokine signaling 3

Figure 2

Changes of TNFα and IRS1 (Ser307) levels in REC. (A) Enzyme-linked immunosorbent assay (ELISA) data for tumor necrosis factor alpha (TNFα) on retinal endothelial cells (REC) in normal glucose (NG, 5 mM) or high glucose (HG, 25 mM) and transfected groups. microRNA (miR)-15b/16 decreased TNFα levels significantly compared to the control HG condition. (B) Western blot results for phosphorylated IRS1 on Ser307 and total IRS1. Increased levels of insulin receptor substrate 1 (IRS-1)Ser307 phosphorylation under hyperglycemic conditions were not changed in REC with miR overexpression. A representative blot is shown. # P < 0.05 versus NG, *P < 0.05 versus HG, N = 3; data are mean ± S.E.M.

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Journal of Neuroinflammation

ISSN: 1742-2094