The levels of SOCS3 and IR (Tyr960) phosphorylation in REC. Western blot results for suppressor of cytokine signaling 3 (SOCS3) (A), and insulin receptor (IR) (Tyr960) and total IR (B) on REC in normal glucose (NG, 5 mM) or high glucose (HG, 25 mM) and transfected groups. (A) The levels of SOCS3 were significantly reduced with overexpression of miR-15b and miR-15b/16 in hyperglycemia. (B) The levels of IRTyr960 phosphorylation in HG were not affected by miR-15b and miR-16 overexpression. A representative blot is shown. #
P < 0.05 versus NG, *P < 0.05 versus HG, N = 4; data are mean ± S.E.M.