Figure 3
From: Endogenous antibodies contribute to macrophage-mediated demyelination in a mouse model for CMT1B
JHD-deficient mice lack B-lymphocytes and antibodies. (A) Immunohistochemical staining against B220-positive B-lymphocytes in wildtype controls and JHD-deficient mice. Note lack of immunoreactivity in JHD-deficient mice. Scale bar, 50 μm. (B) IgG deposition was not detectable in 6-month-old femoral quadriceps (q) and saphenous (s) nerves from JHD-deficient mice. The corresponding phase contrast (PC) microscope image is also shown. Scale bar, 20 μm. (C) Western blot analysis confirms the lack of endogenous antibodies in JHD-deficient mice. ERK1/2 serves as a loading control.