Figure 6
From: Endogenous antibodies contribute to macrophage-mediated demyelination in a mouse model for CMT1B
Antibodies accumulate in peripheral nerves after passive transfer and lead to macrophage increase. (A) Immunohistochemical localization of antibodies after systemic injection of IgG in the femoral quadriceps (q) and saphenous nerve (s) from 6-month-old P0wt JHD−/− (7 days post injection, DPI) and P0het JHD−/− mice (7DPI and 28DPI). Note stronger IgG immunoreactivity in the endoneurium from P0het JHD−/− mice that received consecutive IgG injections over 28 days (28DPI). Scale bar, 20 μm. (B) Quantification of F4/80-positive profiles in non-injected control and reconstituted mice showing an increase of macrophage numbers in femoral quadriceps nerve from P0het JHD−/− mutants after IgG reconstitution (n = 3 to 5). One-way ANOVA with Bonferroni-Holm correction. *P < 0.05. (C) Quantification of foamy macrophages reveals an increase of phagocytosing macrophages in femoral quadriceps nerves from P0het JHD−/− mice after IgG reconstitution. Note also the presence of phagocytosing macrophages in control-reconstituted animals (+KLH, +IgG Fc) (n = 3 to 5). Kruskal-Wallis test with Bonferroni-Holm correction. *P < 0.05. n.d. (not detected).