Co-expression of Iba-1 and M1/M2 polarization markers. Brain slices were prepared at 72 h after MCAO. (A) Sketch picture showing the position of the immunofluorescence pictures obtained from the penumbra of the infarct cortex. (B) Quantification of the percentage of CD16/32+/Iba-1+ cell. (C) Quantification of the percentage of CD206+/Iba-1+cell. (D) Cortex co-stained for CD16/32 (M1 marker) (red) and Iba-1 (green). (E) Cortex co-stained for CD206 (M2 marker) (red) and Iba-1 (green). Images were captured with fluorescence microscope. *
P < 0.05 vs. MCAO-veh; n = 3 per group.