MA increased PPARγ
transcriptional activity. Cortex lysates were from post-MCAO mice brains at 24 h after MCAO. (A) Activation of PPARγ was analyzed by EMSA at 24 h after MCAO using specific PPRE probes as described in the ‘Materials and methods’ section. The abbreviations above the lines represent the following: neg, negative control; sh-v, sham-veh; sh-m, sham-MA; mc-v, MCAO-veh; mc-m, MCAO-MA; cold, positive control + 200-fold cold probe; mut, positive control + 200-fold mutation probe. (B) Quantification of PPARγ level was expressed as the fold change compared to the sham group. (C, D) Binding activity of PPARγ with YM-1 (C) and CD206 (D) promoter after MCAO was detected by ChIP assay with a PPARγ antibody at 24 and 48 h. Quantifications were expressed as the fold change compared to sham group.*
P < 0.05 vs. sham; #
P < 0.05 vs. MCAO-veh; n = 6 in each group.