Figure 2

Toxin-induced ODC death and demyelination leads to increased bioluminescence in oLucR mice. (a) Composite score of clinical disability in DTx-treated oLucR/DTR and control oLucR mice (n = 5). Details of score in [11]. (b) Ratio of mean CNS-specific bioluminescence between DTx-injected oLucR/DTR and oLucR control mice imaged in an IVIS three times a week. Data are representative of two independent experiments (n = 10). The red line indicates the mean baseline photon emission before DTx treatment. The blue bar indicates the period of DTx treatment. (c) Representative pictures of freshly prepared luciferin-bathed brain slices from DTx-treated oLucR/DTR and oLucR control mice 7 weeks p.a. An overlay of photographic picture and photon emission is shown. (d) CNS from DTx-treated oLucR/DTR and control oLucR mice 7 weeks p.a. were dissected, and lysates were analyzed by luminometer (mean ± SEM, n = 4). Gene expression changes in the CNS of oDTR and control mice following DTx treatment. mRNA levels of MOG (e), and β-actin (f) were measured in oLucR/DTR and control mice 9 days after DTx injections (n = 4, mean ± SEM). OB = olfactory bulb; CC = corpus callosum.