increase in CNS-specific bioluminescence during neuroinflammation. (a) Representative freshly dissected luciferin-bathed brain slices from oLucR animals at different time points following MOG immunization. An overlay of photographic picture and photon emission is shown. The EAE score at the time of analysis is shown below the individual pictures. (b) Representative freshly dissociated luciferin-bathed brain slices and whole spinal cords from MOG-immunized animals and not immunized control mice. Overlay of photographic picture and photon emission is shown. (c) CNS from MOG-immunized and control oLucR mice 25 days p.i. were dissected, and luciferase activity within lysates was analyzed by luminometer (mean ± SEM, n = 4).