Figure 5

RT-PCR analysis in the CNS of MOG-immunized versus control mice reveal upregulated expression of cytoskeleton genes. mRNA levels of MOG (a), NG2 (b), and β-actin (c) were measured in MOG-immunized and not immunized (control) oLucR mice at clinical onset of EAE (d) or at onset +5 (mean of EAE/control ratio ± SEM, n = 5). (e) Sagittal sections of spinal cord from mice at disease onset and controls were immunostained with MBP- and actin-specific antibodies. Shown are representative pictures of ODCs in white matter areas (n = 3). Scale bar, 6 μm. (f) Relative mRNA expression in FACS-sorted EYFP⁺ ODCs from the CNS of MOG peptide immunized mice and naïve controls at day of clinical onset of EAE (mean ± SEM, n = 5). Two-tailed Student’s t test, *P < 0.05.