Figure 1

CD14 ⁺ back gating and dot plots of monocyte populations. (A) Backgating of CD14⁺ population shows singlet isolation, identification of viable population, and forward and side scatter properties of parent population. CD14⁺ monocytes are the parent population for all monocyte populations/subsets described in this manuscript. (B) Representative dot plots demonstrate gating strategies used in identifying specific monocyte subsets under the different treatment conditions.