Figure 1

Decrease in CD4 ⁺ CD25 ⁺ cells in MOG35-55-stimulated APC/T _MOG co-cultures is not affected by CBD. Splenocytes (including accessory CD4⁺ T cells) were co-cultured with T_MOG cells and stimulated with MOG35-55 at 5 μg/ml. CBD at 5 μM was added to the cells immediately before MOG35-55. (A) Bar graph showing the average percentage of CD4⁺ cells in APC/T_MOG co-cultures (100% as equal to total number of cells). Neither treatment affected overall CD4⁺ T cell number; (B) dot plot histogram presenting CD4⁺ T cells in APC/T_MOG co-cultures positive and negative for CD25 expression; (C) a representative dot plot showing the changes in CD4⁺CD25⁺ T cell population in control or MOG35-55-treated APC/T_MOG co-cultures in the presence or absence of CBD; (D) bar graph showing the number of cells positive for CD4 and CD25 ± SEM. ANOVA F(3,8) = 7.8, P < 0.01. *P < 0.05 vs non-stimulated cells. n = 3 to 4.