Figure 2

CBD treatment results in upregulation of CD69 and of LAG3 regulatory molecules on CD4 ⁺ CD25 ⁻ T cells in MOG35-55-stimulated APC/T _MOG co-cultures. APC/T_MOG co-cultures were treated with MOG35-55 and CBD. (A) Percentage of CD4⁺CD25⁻ T cells expressing CD69 (ANOVA F(3,12) = 68.9; P < 0.001); (B) percentage of CD4⁺CD25⁻ T cells expressing LAG3 (ANOVA F(3,12) = 13.0, P < 0.001); (C) the representative contour plot density graphs showing the co-expression of CD69 and LAG3 in the CD4⁺CD25⁻ subpopulation; (D) percentage of CD4⁺CD25⁻ cells expressing both CD69 and LAG3 ± SEM. ANOVA F(3,7) = 7.3, P < 0.05. *P < 0.05, **P < 0.01, ***P < 0.001 vs non-stimulated cells; ^# P < 0.05, ^## P < 0.01, ^### P < 0.001 vs MOG35-55-stimulated cells. n = 3 to 4.