Figure 5
Upregulation of MHCII in CD19 high B cells in MOG35-55-stimulated APC/T MOG co-cultures is decreased by CBD. APC/TMOG co-cultures were stimulated with MOG35-55, treated with CBD and the percentage of CD19highMHCIIhigh B cells determined using flow cytometry. (A) Neither treatment affected the number of CD19+ B cells; (B) dot plot showing representative flow cytometry gating of CD19+ B cells expressing MHCII; (C) representative dot plot graph of CD19highMHCIIhigh cells; (D) bar graph showing the mean ± SEM percentage of CD19highMHCIIhigh B cells within the total CD19+MHCII+ cells (taken as 100%). ANOVA F(3,8) = 7.6, P < 0.05. *P < 0.01 vs control, # P < 0.01 vs MOG-stimulated cells. n = 3.