Cannabidiol, a non-psychoactive cannabinoid, leads to EGR2-dependent anergy in activated encephalitogenic T cells

Table 2 CBD treatment does not affect CD69, CD25, and LAG3 expression in resting cells

Phenotype	Control (%)	CBD (%)	T test
Resting splenocytes
CD4⁺	20.0 ± 2.7	20.7 ± 2.2	ns
CD4⁺CD69⁺	6.9 ± 1.2	8.7 ± 1.9	ns
CD4⁺LAG3⁺	0.5 ± 0.3	0.4 ± 0.4	ns
CD19⁺	59.4 ± 2.0	59.4 ± 2.1	ns
CD19⁺CD69⁺	13.6 ± 3.4	17.4 ± 3.4	ns
CD19⁺CD25⁺	2.5 ± 0.2	3.1 ± 0.2	ns
Resting TMOG cells
CD69⁺	0.9 ± 0.3	1.0 ± 0.2	ns
LAG3⁺	0.7 ± 0.2	0.5 ± 0.1	ns

Freshly isolated mouse splenocytes (including accessory CD4⁺ T cells and CD19⁺ B cells) or resting T_MOG cells were maintained separately for 18 h in maintenance medium without MOG35-55 and in the presence or absence of CBD at 5 μM. CD69, LAG3 and CD25 surface expression was analyzed using flow cytometry (n = 3). Data are expressed as percentage ± SEM defined as the percent of cells expressing relevant antigen within an appropriate parent population.
ns, not significant.

ISSN: 1742-2094