Figure 1From: Inflammatory mediator release from primary rhesus microglia in response to Borrelia burgdorferi results from the activation of several receptors and pathways Role of microglial TLR2 receptor in mediating innate immune responses to B. burgdorferi. Primary microglia were incubated with 25 nM TLR2 or a non-specific control (CTL) siRNA (described in the ‘Methods’ section) followed by infection with B. burgdorferi for 24 h. Supernatants were collected at 24 h post addition of the spirochetes and assayed for cytokine and chemokine levels by a multiplex ELISA. Data are representative of three experiments using microglia from two animals. Pam3CSK4 was used as a positive control and cell culture medium as the negative control. Bars represent standard deviation. *P < 0.05; **P < 0.01; ***P < 0.001. CCL2, chemokine (C-C) motif ligand 2; CXCL8, chemokine (C-X-C) motif ligand 8; IL-6, interleukin 6; siRNA, small interfering RNA; TLR, Toll-like receptor; TNF, tumor necrosis factor.Back to article page