Figure 3

Role of TLR5 in immune mediator production from NHP microglia in response to B. burgdorferi. A concentration of 25 nM TLR5 or CTL siRNA was used to determine the role of microglial TLR5 receptor in mediating inflammatory responses to B. burgdorferi. A pattern of inhibition was determined from four experiments performed with microglia obtained from two animals. A representative pattern is shown, with bars portraying standard deviation. FliC was used as a positive control for siRNA efficacy, and the medium alone was used as a control for basal expression of inflammatory mediators. *P < 0.05; **P < 0.01; ***P < 0.001. The inset figure shows magnification of the data within the square at the bottom. CCL2, chemokine (C-C) motif ligand 2; CTL, control; CXCL8, chemokine (C-X-C) motif ligand 8; IL-6, interleukin 6; siRNA, small interfering RNA; TLR, Toll-like receptor; TNF, tumor necrosis factor.