Figure 5From: Inflammatory mediator release from primary rhesus microglia in response to Borrelia burgdorferi results from the activation of several receptors and pathways Role of NOD2 receptor in mediating inflammatory molecule release from microglia exposed to B. burgdorferi. Primary microglia were co-incubated with 15 nM NOD2 or CTL siRNA for 24 h, followed by B. burgdorferi infection for an additional 24 h. Supernatants were collected 24 h post infection and analyzed for chemokine and cytokine expression. A representative pattern from five independent experiments from four animals is shown. Bars represent standard deviation. NOD2 ligand MDP was used as a positive control for NOD2 mediated effects. *P < 0.05; **P < 0.01; ***P < 0.001. CCL2, chemokine (C-C) motif ligand 2; CTL, control; CXCL8, chemokine (C-X-C) motif ligand 8; IL-6, interleukin 6; MDP, muramyl dipeptide; NOD2, nucleotide-binding oligomerization domain containing 2; siRNA, small interfering RNA; TNF, tumor necrosis factor.Back to article page