Figure 6

Efficacy of NOD2 siRNA in suppressing NOD2 protein expression. RNAi experiment was carried out according to the protocols described in the ‘Methods’ section, with 25 nM control or NOD2 siRNA and 2.5 μg/mL of MDP. Following fixation in paraformaldehyde, cells were analyzed for NOD2 expression by immunofluorescence. (A) shows cells (assigned red) stained for NOD2 (green fluorescence) with bars representing 25 μm, and (B) shows the percent change in NOD2 expression in the two treatment groups, with vertical bars representing standard deviation. Data was collected from two experiments conducted on microglia collected from a single animal.*P < 0.05. CTL, control; MDP, muramyl dipeptide; NOD2, nucleotide-binding oligomerization domain containing 2; siRNA, small interfering RNA.