Inflammatory mediator release from primary rhesus microglia in response to Borrelia burgdorferi results from the activation of several receptors and pathways

Table 2 Average fold change ^a in chemokine/cytokine expression in the presence of 25 to 30 nM NOD2 siRNA

Cytokine or chemokine/treatment	IL-6	CXCL8	CCL2	TNF
B. burgdorferi	0.817^b	1.165^c	0.803^b	0.703^b
B. burgdorferi	(±0.041)	(±0.117)	(±0.118)	(±0.116)
MDP	0.7034^b	1.0295	0.6889^b	0.6486^b
MDP	(±0.170)	(±0.115)	(±0.062)	(±0.494)

^aFold changes in chemokine/cytokine expression were calculated as treatment with control siRNA/treatment with NOD2 siRNA. Values for B. burgdorferi (MOI 10:1) treatment were calculated from three experiments using microglia from three different animals; MDP (2.5 μg/mL) was from four experiments from four animals. Values higher than 1.0 represent fold-down regulation of chemokine/cytokine while values less than 1.0 represent an increase. Standard error of the mean (SEM) is indicated within parentheses. ^bA statistically significant increase in expression. ^cValues that were significantly downregulated in the majority of experiments (≥67%). chemokine (C-C) motif ligand 2; CXCL8, chemokine (C-X-C) motif ligand 8; IL-6, interleukin 6; MDP, muramyl dipeptide; TNF, tumor necrosis factor.

ISSN: 1742-2094