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Figure 1 | Journal of Neuroinflammation

Figure 1

From: EP2-PKA signaling is suppressed by triptolide in lipopolysaccharide-induced microglia activation

Figure 1

Triptolide suppresses NO production and iNOS expression in LPS-activated microglia. (A) Triptolide reduced NO production in BV2 cells. BV2 cells were stimulated with LPS (0.01 μg/mL) in the presence or absence of different concentrations of triptolide (0, 12.5, 25, 50 nM) for 24 h. At the end of the incubation period, the supernatants were collected for NO measurements. (B) Triptolide reduced NO production in primary rat microglial cells. Primary rat microglial cells were stimulated with LPS (0.01 μg/mL) in the presence or absence of triptolide (0, 12.5, 25, 50 nM) for 24 h. At the end of the incubation period, the supernatants were collected for NO measurements. (C) Triptolide reduced iNOS expression in primary rat microglial cells. Primary rat microglial cells were stimulated with LPS (0.01 μg/mL) in the presence or absence of triptolide (0, 12.5, 25, 50 nM) for 24 h. The cells were then lysed to detect iNOS expression by Western blot. (D) The protein levels were quantified relative to GAPDH levels and normalized to the LPS-stimulated group. ***P < 0.001. # P < 0.05, ## P < 0.01, and ### P < 0.001.

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