Figure 3

EP2 signaling pathway regulates NO release in primary rat microglial cells. (A) EP2 expression in primary rat microglial cells. Immunofluorescent staining of EP2 and CD11b, as well as the nuclear stain DAPI are shown (scale bar, 25 μm). (B) cAMP accumulation in rat microglia cells left untreated or treated with butaprost (10 μM) or AH6809 (10 μM) for 3 h (n = 3). (C) Butaprost stimulated NO production in resting microglia. Primary rat microglial cells were stimulated with different concentrations of butaprost (0, 0.1, 1, 10 μM) for 24 h. At the end of the incubation period, the supernatants were collected for NO measurements. (D) AH6809 suppressed NO production in LPS-activated microglia. Primary rat microglial cells were unstimulated or stimulated with LPS (0.01 μg/mL) in the presence or absence of AH6809 (0.1, 1, 10 μM) for 24 h. At the end of the incubation period, the supernatants were collected for NO measurements. ***P < 0.001 and ^## P < 0.01.